International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 6 (2013) pp. 236-245
Isolation and Identification of Actinomycetes Isoptericola variabilis From Cauvery River Soil Sample
M.Rajesh Muthu1 , R.Subbaiya1* , M.Balasubramanian1 , P.Ponmurugan1 and Masilamani Selvam2
1Department of Biotechnology, K.S.Rangasamy College of Technology, Tiruchengode-637 215, Tamil Nadu, India. 2Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Chennai-600 019, Tamil Nadu, India *Corresponding author e-mail:
Five actinomycetes strains were isolated from the 25 soil samples of Cauvery river. The isolated strains were subjected to different biological identification. Collected soil samples were air dried for 2-4 days. After 4 days of air drying it has been washed two times with the help of distilled water to remove the unwanted particles from the soil sample. The pure sample was subjected to basic biological procedures like serial dilution, pour plate technique and spread plate technique, that is to know the specific growth of the actinomycetes. Isolated actinomycetes culture was identified by 16s rRNA sequencing and it was confirmed by using bioinformatics tool as BLAST (Isoptericola variabilis).
Actinomycetes; BLAST; Isoptericola variabilis.