International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 6 (2013) pp. 228-235
Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial and antioxidant property
A.Lalitha, R.Subbaiya* and P.Ponmurugan
Department of Biotechnology, K.S.Rangasamy College of Technology, Tiruchengode-637 215, Tamil Nadu, India *Corresponding author e-mail:
The field of nanotechnology is one of the most active researches nowadays in modern material science and technology. Eco friendly methods of green mediated synthesis of nanoparticles are the present research in the limb of nanotechnology. The present work leads to the synthesis of nanoparticles from 1mM AgNO3solution through aqueous leaf extract of Azhadirachta indica as reducing as well as capping agent. Synthesized nanoparticles are characterized under UV-Vis spectroscopy at the range of 350-420nm. The peak showed at 351nm. Further this range and size was confirmed by Particle Size Analyzer. The chemical groups studied using FT-IR analysis. Green synthesized silver nanoparticle showed zone of inhibition against isolated Gram positive (Salmonella typhi) and Gram negative (Klebsiella pneumoniae) bacteria. The leaf extract shows higher antioxidant activity found by DPPH assay and Hydrogen Peroxide assay.Based on the result obtained it can be said that the plant resources can efficiently used in the prouction of silver nanoparticle and it could be utilized in various fields such as biomedical, nanotechnology and so on.
Silver nanoparticle; FT-IR,DPPH; antioxidant property; Azhadirachta indica.