International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 6 (2013) pp. 204-214
Protein Profile Analysis of Escherichia coli O157:H7 from Human and Animals Origin
I Wayan Suardana1*, Komang Januartha Putra Pinatih2 , Ni Luh Ketut Ayu Ratnawati3 , Dyah Ayu Widiasih4
*1Department of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University, Denpasar. Jl. PB.Sudirman Denpasar-Bali, Indonesia 2Department of Microbiology, Faculty of Medicine, Udayana University Denpasar, Jl. PB. Sudirman Denpasar-Bali, Indonesia 3Publich Health Centre I, Department of Health, Badung District-Bali 4Department of Veterinary Public Health Faculty of Veterinary Medicine, Gadjah Mada University, Jl. Fauna 2, Karang Malang, Yogyakarta 55281, Indonesia .*Corresponding author e-mail:
Healthy animals especially of the type of ruminants (cattle, sheep, and goats) and pigs are commonly carrying a large number of STEC and Escherichia coli O157:H7 in their feces, so they were considered as a main reservoir of the agent. The transmission of this agent to human commonly by consumption of less cooking meat, unpasteurized dairy product, water contaminated by feces, as well as person to person transmission has also been reported. Based on the importance of this agent to human healthy especially to the epidemiological aspect in order to know the pathogenesis of the agent, the genetic variation study of isolates originated from animals and human needs to be done. The study began by the cultivation of 20 E. coli O157:H7 isolates originated from animals and human, followed by analysis of the chemical characterization using SDS-PAGE (Sodium Dodecyl Sulphonate Polyacrylamide Gel Electrophoresis) method. The data from SDS- PAGE were analyzed using simple matching coef icient (Ssm) and algorithm unweighted pair group method using arithmetic averages (UPGMA). The results show that the protein profile of most E. coli O157:H7 local isolates are known to have similarity value more than 70%. These results prove that the isolates of animal origin can be found in humans or nature zoonotic.
E. coli O157:H7; SDS-PAGE; human; animals; zoonotic