International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 6 (2013) pp. 146-152
Identification of indoor airborne microorganisms in residential rural houses of Uttarakhand, India
Manisha Joshi* and R.K. Srivastava
Department of Environmental Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar- 263145, Uttarakhand, India. *Corresponding author e-mail:
Indoor air contains a complex mixture of microorganisms, microorganism fragments, and by products such as molds, bacteria, endotoxins, mycotoxins, and volatile microbial organic compounds. Airborne bacteria can be toxic, allergenic, and/or infectious. This research attempts to uses a molecular approach for detecting microbes and fungi found in indoor environment of residential houses. In this study three bacterial and three fungal species has been isolated from sample collected from indoors in rural residential house of Uttarakhand. A Polymerase chain reaction (PCR) based method for the specific detection of microbes was developed. DNA isolated from collected sample served as a template for PCR amplification with specific primer pairs targeting the 16S rRNA gene. The PCR amplification products were sequenced and the obtained sequences were analyzed. The results of this work confirm the presence of the Brevibacillus brevis, Arthrobacter sp. FB24 and Bacillus cereus bacterial species and Neosartorya fischeri, Aspergillus clavatus and Trichoderma reesei fungal species in indoor air.
Indoor air; Fungal and bacterial species; DNA isolates; Polymerase chain reaction (PCR)