International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 52-67
Rapid decolourization of Disperse Red F3B by Enterococcus faecalis and its Phytotoxic Evaluation
S.Senthil Kumar1*, T.Muruganandham1 , V.Kathiravan1 , R. Ravikumar2 and M.S. Mohamed Jabbir1
1PG and Research Department of Biotechnology, National College (Autonomous), Tiruchirapalli 620 001, Tamil Nadu, India 2PG and Research Department of Botany, Jamal Mohamed College (Autonomous), Tiruchirapalli 620 020, Tamil Nadu, India *Corresponding author
Rapid decolourization potential of a novel bacterial isolate was developed from a mixed culture isolated from a textile industrial ground at Mettuppalayam, Tamil Nadu, India. The isolate decolorized Disperse Red F3B to 94% at 100 mg l-1 concentration within 5 hrs under microaerophilic condition at 50°C. The bacterial isolate was determined to be Enterococcus faecalis based on the biochemical characteristics and 16S rDNA sequence analysis. Dye decolourization was optimum in Nutrient broth and the biotransformation was confirmed through Ultra Violet-Visble Spectroscopy, Thin Layer Chromatography and Fourier Transformed Infra Red spectroscopy analysis of the initial, intermediate and final decolorized sample. Phytotoxicity testing with the seeds of Brassica nigra and Cyamopsis tetragonolobus demonstrated more sensitivity towards the native dye, while the products after decolourization did not have any inhibitory effect as seen from germination delay index and morphometric parameters
Biodegradation; azo dye; germination delay index; Brassica nigra; Cyamopsis tetragonolobus.