International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 436-440

Virulence-associated factors in Escherichia coli strains isolated from urinary tract infections

P. Sai Swaroop, P. Hema Prakash Kumari*, and U. Sreenivasa Rao

Department of Microbiology ASRAM Medical College, Eluru, A.P Pin 534004, India

ABSTRACT

To study the occurrence of Uropathogenic Escherichia coli (UPEC) in cases with urinary tract infections. A total of 75 cases from urinary tract infections and 35 stool samples from apparently healthy individuals were included. The colonies identified as Escherichia coli were screened for virulence factors namely haemolysin, Mannose Resistant and Mannose Sensitive Haemagglutination (MRHA, MSHA) by recommended methods. Among 75 cases 30 (40 %) were haemolytic, 21 (28%) showed MRHA, 23 (31%) showed MSHA. In 35 controls 3(6%) were haemolytic, 2 (5.71 %) showed MRHA, 9 (18%) and 3 (8.57%) showed MSHA. The difference between cases and controls for haemolysis and MRHA were significant (p<0.001 and p<0.01 respectively). Out of 75 urinary isolates, 44 (58%) could be labelled as UPEC.

Keywords

UPEC; Stool samples; E.coli; MRHA; MRSA.