International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 418-427
DNA Based Identification and Characterization of Thermophilic Streptomyces sp. From Desert Soil of Rajasthan
Dimple Songara* and Swarnjeet Kaur
Karanja House 2, Dumayne road, Colaba, Mumbai, India
The present study was aimed at to identify the thermal resistant actinomycetes from the desert soil of Rajasthan, India. The morphology, cultural, physiological, biochemical and phylogenetic analysis of isolate stb-1 were characterized. According to the morphology, isolate stb-1 was identified as a representative of the genus Streptomyces. The 16S rRNA region of this strain was amplified and sequenced. A Neighbour-Joining algorithm tree of 16S rRNA was constructed. The strain showed growth at high temperatures and were screened for antagonistic potentiality against gram positive and gram negative human pathogenic bacteria. The strain stb-1 showed broad spectrum antibiotic activity against the tested pathogens. According to the observation and phylogenetic analysis, the strain stb-1 was proved to belong to the genus Streptomyces with thermal resistance and antibiotic potentiality.
Actinomycetes; morphology phylogeny; antibacterial; Streptomyces; thermal resistance; 16s r RNA