International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 353-368
Evaluation of Antibacterial Potential of Selected Plant Extracts on Bacterial Pathogens Isolated from Urinary Tract Infections
Hajera Tabassum1* , Mir Naiman Ali2 , Noura Al-Jameil1 and Farah Aziz Khan1
1College of Applied Medical Sciences, King Saud University, Saudi Arabia 2Department of Microbiology, Mumtaz Degree & P.G.College, India
In the present study 200 urine samples were collected from both male and female patients suffering with urinary tract infections (UTI). A total of 75 bacterial cultures were isolated belonging to 5 species: Escherichia coli (44%); Klebsiella pneumoniae (25.33%); Pseudomonas aeruginosa (20%); Enterobacter faecalis (6.66%) and Proteus mirabilis (4%). Six plants (Coriander sativum, Syzygium aromaticum, Cinnamomum cassia, Zingiber of icinale, Terminalia chebula and Azadirachta indica) and their parts (leaves, bark, flower, rhizome and fruit) were used to evaluate their antibacterial potential. Aqueous, methanolic and ethanolic extracts were used for studying antibacterial activity by agar well diffusion assay and Minimum inhibitory concentration method. Among the three extracts used, highest antibacterial activity was recorded with ethanolic extracts of Cinnamomum cassia on E. coli and least against K. pneumoniae with diameter of inhibition zones (DIZ) of 21.33 ± 0.57 and 15.66 ± 0.57 mm respectively. Preliminary phytochemical analysis of the plant parts revealed the presence of active compounds such as phenolics, tannins, alkaloids and flavonoids. The results obtained in this study clearly demonstrated higher and broad spectrum antibacterial activity of selected plant extracts on all five UTI isolates compared with ten standard antibiotics used for treating UTI.
Urinary tract infection; antibacterial activity; phytochemicals; minimum inhibitory concentration