International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 347-352
Production and Optimization of Citric Acid by Aspergillus niger using Fruit Pulp Waste
Pratik Bezalwar*, Ashok V. Gomashe, Harshal M.Sanap and Pranita A. Gulhane
Department of Microbiology, S.S.E.S.A s Science College, Congress Nagar, Nagpur-440012 (MS), India
Citric acid is a weak organic acid, which is used in many pharmaceutical and in other industrial food products. Increased demand of citric acid has led to search for high yielding fermentable strains of microorganisms and cheaper fermentation substrate in many countries. The present study suggests that in near future waste fruit pulp could be one of the substrate for citric acid production at industrial scale worldwide. Production of citric acid was carried out by Aspergillus niger in the medium without methanolas well as withmethanol. Maximum citric acid production was obtained by using pineapple pulp waste i.e., 3.25 g/kg of citric acid in the medium without methanol and5.25g/kg with methanol. Citric acid production was found to be2.75, 2.63, 1.75, and 1.5and 0.75g/kg of the substrate in the medium without methanolwhile, 0.16, 0.13, 0.19, 0.15 and 0.22g/kg of the substrate with methanol by using dry fruit pulp of Indian jujube, Beet root apple, Guava,Papayaand Wood apple respectively.
Citric acid; Aspergillus niger; fruit pulp waste