International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 293-299
Prevalence of Methicillin-Resistant Staphylococcus aureus colonisation and its antibiotic susceptibility profile among healthcare personnel in a tertiary care setup of northern India
Amit Kumar Singh*, Mahendra Gupta, Anjali Agarwal, Prashant Gupta and Mastan Singh
Post-graduate Department of Microbiology, King George s Medical University, Lucknow, UP, India
Methicillin resistant Staphylococcus aureus (MRSA) is one of the most common nosocomial pathogen. Colonisation of MRSA among the health care personnel (HCP) taking care of patients may lead to spread of disease among patients and co- workers. The present study is aimed to detect the methicillin resistance in Staphylococcus aureus isolates obtained from the health care staff from selected wards of a 2800-bedded tertiary care hospital setup of northern India. Nasal and hand swabs collected from health care personnel of Cardiothoracic and vascular surgery (CTVS) wards and Trauma ventilatory units (TVU). Detection of methicillin resistance was performed for Staphylococcus aureus isolated from the swabs as per CLSI guidelines using cefoxitin 30µg disc. Out of 166 health care personnel, Staphylococcus aureus isolates were detected in 76/166 (45.78%) and MRSA was detected in 22/166 (13.25%). Of the 22 HCP with MRSA, 2/22(9.09%) were carrying MRSA in both hand and nasal swabs, whereas 12/22(54.54%) were only nasal carriers and 8/22(36.36%) were only hand carriers. Rate of colonisation of Staphylococcus aureus among HCP was 45.78% and carriage of MRSA was 13.25% and thus the possibility of spread of MRSA among the patients in the hospital
MRSA; HCP; CTVS; TVU. Staphylococcus aureus; cefoxitin.