International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 142-149
Isolation, characterization of phytase producing Bacillus sps NBtRS6 from the rhizospere soil of NBt cotton field
K.Ushasri*, P.Sivaragini and .K.Vijayalakshmi
Department of Applied Microbiology, S.P.M.V.V. Tirupati - 517 502 A. P. India
Soil samples of Bt Rhizosphere were collected from NBt cotton growing area of Andhra Pradesh, India and was used as a source material for isolation and screening of phytase producing bacteria. 21 Bacteria were isolated from NBt Rhizosphere. Phytase enzyme activity of the cultures was screened on Modified phytase solubulizing medium (MPSM). The result inferred that six isolates NBtRS1 to NBtRS6 were strongly positive in enzyme activity than six of other microorganisms while nine isolates were found negative and among all ,Isolate NBtRS6 produced significantly higher phytase yield than other isolates and was chosen for species identification. Preliminary identification by microscopic and biochemical tests identified the isolate NBtRS6 as Bacillus sp and designated as Bacillus sp NBtRS6.