International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 126-134
Molecular studies on chitinase producing, biofilm forming Pseudomonas putida isolated from fish waste dumping yards
N. Saranya1* , and Tha. Thayumanavan2
1Department of Biotechnology, Karpagam University, Coimbatore, India 2School of Biotechnology, Dr. G.R. Damodaran College of Science, Coimbatore, India
Dual action of the Pseudomonas putida forming antibiotic and biofilm in same species is useful for the plants against the fungal pathogens. lapD, a gene which is located flanking to the lapA gene, which is possible role in controlling lapA protein secretion and thus plays a major role in biofilms formation in Pseudomonas sp. Pyocyanin is a bioactive compound, produced by majority of the Pseudomonas sp. In this study, the screening of lapD (biofilm gene) gene and phzS (pyocyanin gene) among the P. putida isolated from fish waste dumping locations. Being a potential biofilm former, all the isolates of P. putida were tested for the presence of lapD gene, since it role is highly significant in biofilm formation and phzS gene for it antibiotic activity. Among the 15 isolates tested, only one is positive for lapD gene, while 4 isolates were having pyocyanin gene (phzS).
P. putida; biofilm; pyoyanin; lapD; phzS.