International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:9, September, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 2857-2864

Molecular Survey of Tobacco Leaf Curl Virus Disease in Different Parts of Karnataka
Gurudevi V. Navali* and K.T. Rangaswamy
Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru-560065, Karnataka, India
*Corresponding author

Surveys were conducted during 2016-18 revealed that the occurrence of the leaf curl disease on tobacco in major growing districts of Karnataka ranged from 19.60 to 31.01 during 2016-18. Among the six districts surveyed, highest per cent disease incidence of tobacco leaf curl virus was recorded in Shivamogga district (31.01) followed by Belagavi (25.59) and lowest per cent disease incidence of 19.60 of TbLCV was recorded in Chamarajangar district. The survey data imply that leaf curl disease is emerging as one of the major diseases of tobacco. For the detection of the virus, total DNA was extracted and PCR was carried out using pair of begomovirus specific Deng primers. All the isolates collected at different locations showed characteristic band of 550 bp whereas no amplicon was observed in healthy tobacco samples. This indicating the begomovirusis involved in causing the leaf curl disease in tobacco.

Keywords: Tobacco, Leaf curl disease, Survey, Detection

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How to cite this article:

Gurudevi V. Navali and Rangaswamy, K.T. 2018. Molecular Survey of Tobacco Leaf Curl Virus Disease in Different Parts of Karnataka.Int.J.Curr.Microbiol.App.Sci. 7(9): 2857-2864. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.