National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A study was carried out on semen of nine healthy mature breeding bulls (3 each of Gir, Surti and Murrah breed) to evaluate the correlations of their fresh sperm quality attributes to those of frozen thawed semen assessed both by subjective method and objective technique using Biovis CASA. The ejaculates immediately after collection were evaluated for routine seminal attributes. The ejaculates (n=72, 24 of each breed) having >75% initial motility were diluted @ 80 million sperm/ml using TFYG extender and were assessed for motility, morphology, viability and hypo-osmotic swelling test (HOST) subjectively, and for motion characteristics by Biovis CASA. The extended semen was filled in French mini straws and frozen in liquid nitrogen using a programmable biofreezer after 4 hrs of equilibration, and was thawed in water bath at 37°C for 30 sec. The correlations of fresh sperm quality and velocity/kinematic parameters of progressively motile sperms to those of post-thawed semen revealed that the motility, live sperm, abnormal sperm, rapid progressive motile sperm, average path velocity (VAP), straight line velocity (VSL) and straightness (STR) of fresh sperm had practically no significant correlations with any of the attributes of its post-thawed semen in any of the three breeds studied. However, the other traits, viz., HOST, total motile sperm, curvilinear velocity (VCL), linearity (LIN), wobbling (WOB), beat-cross frequency (BCF), amplitude of lateral head displacement (ALH), and dancing mean (DNM) of fresh sperm showed significant positive or negative correlations with post-thaw CASA parameters in all three breeds (r = 0.41 to 0.68). Thus, CASA analysis of fresh semen for later traits could predict the post-thawed sperm kinematics of bovine semen.
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