National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The pharmacokinetics, antibacterial and anti-inflammatory activities of Chrysin (100 mg/kg) were studied following intramuscular administration in rats. Drug concentration in rat plasma was determined using High Performance Liquid Chromatography (HPLC). The mean peak plasma drug concentration of 0.24 ± 0.01mg/mL was achieved at 0.25 h. The pharmacokinetic parameters like elimination half-life (t1/2β), apparent volume of distribution (Vdarea) and total body clearance of Chrysin were 0.52±0.03 h, 338.63±13.39 L/kg and 456.20±15.62 L/h/kg respectively were determined. In vitro and in vivo antibacterial activity of Chrysin was determined by microbroth dilution technique against different bacterial pathogens and in neutropenic rat intraperitoneal infection model, respectively. In the present study, Chrysin was found to have no in vitro antibacterial activity in range of 10 - 0.07 mg/mL. In in vivo bacterial colony count between test drug and positive drug (Chloramphenicol) indicated that Chrysin had no protective activity against Staphylococcus aureus in neutropenic rat intraperitoneal infection model. In the present study, Chrysin found to inhibit LPS induced nitric oxide production on RAW 264.7 macrophage cell line and COX-2 enzyme through ELISA method but significantly (p<0.01) lower to Meloxicam. In addition to this Chrysin (100 mg/kg) was found to be effective (34.67 ± 1.55 %) in carrageenan-induced paw edema assay in rat after 4h following intramuscular administration.
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