An experiment was carried out during 2017-18inHorticulture Research Station, Bhubaneswar under Department of Fruit Science & Horticulture Technology, College of Agriculture, Orissa University of Agriculture & Technology to find out the effect of pruning and plant growth regulator like GA3, IAA, NAA, 2,4-D on plant growth and fruit yield rejuvenated sapota plant. Variation due to divergence in genotypes was found significant so far as the morphometric is concerned highest leaf chlorophyll content (27.85SPAD), canopy spread (11.988m in E-W and 10.51m in N-S directions), number of flowers per shoot (9.61), fruit drop (60.406%) were observed in unpruned tree. Whereas maximum leaf area (47.616cm2), fruit set (13.498%) were observed in primary branch pruned trees. Among the growth regulators and NAA@50ppm produced highest leaf area (45.974cm2), no. of flowers per shoot (8.886), fruit set (15.032%), no. of fruits per plant (119.4), yield (14.906kg/tree) Whereas GA3@20ppm produced highest chlorophyll content (25.46SPAD), canopy spread (9.814m in E-W & 8.808m in N-S), fruit weight (134.494g). Hence it is concluded that growth regulator NAA@50ppm influences the yield attributes like fruit set and fruit retention. The treatment combination tip clipping of terminal shoots followed by spraying of NAA@50ppm produced highest fruit yield (19.48kg/tree) and was the best among all other treatment.

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