National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Avian poxviruses cause pox in different kind of birds. In the present study, investigations of a cutaneous form of pox in pigeons were reported. In June 2017, a Pigeon showing clinical lesions of yellowish crust/nodules on and around beak and eyes and feet was observed in a scattered group of around 40 pigeons. Lesions were collected aseptically in a container with 50% glycerol phosphate buffer solution. After processing of clinical specimen the inoculums were inoculated into chicken embryonated eggs by using chorioallantoic membrane route. Viral deoxyribonucleic acid was extracted and used in Avipox virus specific polymerase chain reaction. No specific lesions were observed in inoculated chicken embryonated egg even after third passage. However, in polymerase chain reaction Avipox virus specific P4b gene (578 bp) was successfully amplified and visualized in 1% agarose gel on electrophoresis. Clinical signs and amplification of Avipox virus specific gene confirmed cutaneous form of Pox in Pigeons at Mhow in Madhya Pradesh.
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