LA hybrids of Lilium are a high value ornamental flower having high demand in world cut flower market. The present experiment on Lilium cv. Pavia was conducted at Indian Agricultural Research Institute, New Delhi with an objective to regenerate plantlets from bulb scale by tissue culture technique and further to check the genetic fidelity of the in vitro regenerates. Direct adventitious shoot bud formation was recorded without undergoing any callus phase which was induced from the base of the inner scale. Maximum shoot proliferation was observed with the treatment combination MS + 6-BAP (2 mg/l) + NAA (0.1 mg/l) when transferred to the proliferating media. 6-BAP was found to be more effective than kinetin in shoot multiplication and ranged from 5.68 to 15.80. Twenty in vitro regenerates were randomly selected from the population raised from bulb scale for testing its genetic fidelity using SSR markers. Out of 18 markers screened, only 10 produced clear and reproducible bands. A total of 273 bands were generated from the 10 SSR markers in 21 in vitro regenerated plants. The number of scorable bands for each primer varied from 1 to 2. No Polymorphism Information Content was recorded among the in vitro regenerates. Further, Dendrogram generated clearly indicated that there was no genetic variation among the in vitro plantlets raised from bulb scales. The banding profile of the micro raised plants was monomorphic and similar to the mother plant. Thus, bulb scale can be commercially used for mass multiplication of LA Lilium cv. Pavia.

Download this article as