National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was taken up to evaluate the antibiogram against different mastitogenic pathogens isolated from the milk samples of clinical bovine mastitis cases. A total of 78 milk samples were collected from different dairy farmers of Nialiblock, Odisha and processed in Animal Disease Research Institute (ADRI), Phulnakhara, Cuttack for isolation, identification and antibiogram of pathogens. The samples were screened for mastitis by modified California mastitis test (MCMT).The milk samples were inoculated in BHI broth and incubated for 24-48 hours. Then a loopful of culture was streaked into EMB Agar, Mannitol Salt Agar and Blood Agar. The isolates were identified according to the cultural, morphological and biochemical characteristics as per standard methods. All the isolates were subjected to Kirby-Bauer disc diffusion methods using different antibiotics. Out of 78 samples, 73 samples were revealed to be positive for bacterial growth. Bacterial screening of samples revealed that 22 samples yielded pure culture and 51 samples yielded mixed cultures. The predominant bacterial isolates recovered were Staphylococcus spp (48.17%) followed by E.coli (27.73%), Streptococcus spp (12.41%), Klebsiella spp (9.48%) and Bacillus spp (2.19%).it was found that Staphylococcus is highly sensitive to Enrofloxacin and Gentamicin followed by Ceftriaxone/ Tazobactam and Ciprofloxacin. The study revealed that E. coli is highly sensitive to Chloramphenicol followed by Enrofloxacin, Ciprofloxacin and Gentamicin. The Streptococcus spp is found to be sensitive to Enrofloxacin followed by Ciprofloxacin and Gentamicin. All the isolates are found to be almost resistant against Penicillin G, Oxytetracycline, Ampicillin and Ampicillin/Cloxacillin.
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