National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Present study was undertaken to evaluate the anti-apoptotic effect of LEHD-FMK (caspase inhibitor) on buffalo bull sperm before and after semen freezing. Tris egg yolk extender was treated with Z-LEHD-FMK (0, 2, 4, 6, 10 and 20 µM) and used for extension of six aliquots of semen from each ejaculate to final concentration of 80 million sperm/ml. Sperm samples were analyzed for sperm motility, viability, plasma membrane integrity, mitochondrial membrane potential and PLA (Phospholipase) activity at pre-freeze and post thaw stages. There was no effect (P>0.05) of this caspase inhibitor on percent sperm motility, viability, plasma membrane integrity, active mitochondria and sperms with low PLA (Phospholipase) activity before semen freezing. However, Z-LEHD-FMK (2µM) treated sperm showed higher (P<0.05) motility with functionally intact membrane at post thaw stage as compared to control (0 µM). Percent post thaw viable sperm were higher (P<0.05) in Z-LEHD-FMK (2, 4 and 6 µM) treated semen sample as compared to control. Percent sperm with active mitochondria and low PLA activity were increased in dose dependent manner (P<0.05).
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