International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:9, September, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 702-709

Study of Nosocomial Infections and Molecular Diagnosis of Bacterial Resistance in Patients Admitted in Intensive Care Units of Regional Cancer Center
Foram Maulin Patel*
Department of Microbiology, The Gujarat Cancer & Research Institute, New civil hospital, Asarwa, Ahmedabad, Gujarat, India
*Corresponding author

Nosocomial infection (NI) or health care associated infection is defined as an infection developing in hospitalized patients after 48 hrs, neither present nor in incubation at the time of their admission. The objectives of this study were to determine the frequency and pattern of nosocomial infection in patients admitted in ICU of tertiary cancer hospital and to detect the etiological agent with their antimicrobial resistance by molecular methods and also potential source of infection. Methods: This was an observational study conducted from January 2014 to March 2014. Total 100 different types of samples were collected from 330 admitted patients who developed clinical evidence of infection after 48 hrs of admission in ICUs. Organisms causing infections were identified and they were further subjected for Antibiotic susceptibility testing by MIC and molecular diagnosis of bacterial resistance using reverse hybridization technique. Results: During the study periods, 100 (30.3%) out of 330 patients acquired nosocomial infection. Wound infection was seen in 49 %, followed by respiratory tract infection in 19 % and blood stream infection in 16%, other infections were urinary tract and gastrointestinal infections. Antibiotic resistance profile revealed that majority of bacterial isolates was resistance to multiple antibiotics. Different types of resistance mechanisms were observed in isolated organisms by molecular methods (reverse hybridization). Amongst Gram negative pathogens, 40.90% were ESBL producers, 6.81% were positive for Carbapenamases production. 25% of Gram positive cocci were MRSA positive. Conclusion: Patients admitted in ICUs are at higher risk of acquiring nosocomial infection. Isolated pathogens are multidrug resistant. Standard guidelines for infection prevention should be followed in ICU to reduce the nosocomial infection.

Keywords: Nosocomial infection, Intensive care unit

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How to cite this article:

Foram Maulin Patel. 2018. Study of Nosocomial Infections and Molecular Diagnosis of Bacterial Resistance in Patients Admitted in Intensive Care Units of Regional Cancer Center.Int.J.Curr.Microbiol.App.Sci. 7(9): 702-709. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.