International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:9, September, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 503-512

Effect of Biosynthesized Zinc Oxide Green Nanoparticles on Pulse Beetle, Callosobruchus analis (Coleoptera: Chrysomelidae)
Wazid1*, Sushila Nadagouda1, A. Prabhuraj1, R. Harishchanra Naik1, N.M. Shakuntala2 and H. Sharanagouda3
1Department of Agricultural Entomology, College of Agriculture, UAS, Raichur, Karnataka, India
2Department of Seed Technology, College of Agriculture, UAS, Raichur, Karnataka, India
3Department of Processing and Food Engineering, CAE, UAS, Raichur, Karnataka, India
*Corresponding author

Among the stored product insects, Callosobruhus analis (Fabricius) is a cosmopolitan and serious pest of pulses in field as well as in storage condition. The excessive use of pesticides and chemical compounds led to development of resistance, resurgence and has an adverse effect on environment. This experiment was conducted at Centre for Nanotechnology Laboratory, UAS, Raichur. In the recent years, nanotechnology has emerged as a promising tool for pest control. In the present study, we reported the biological synthesis of zinc oxide green nanoparticles from spinach leaves. The green synthesized zinc oxide nanoparticles were characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), Zetasizer and Scanning electron microscope (SEM). The bio-physical characterization revealed that the zinc oxide nanoparticle has a hexagonal structure with a mean particle size of 20.67 nm. The pesticidal effects of Zinc oxide green nanoparticles (ZnO) were used as stored product insect protectants compared to chemical malathion as standard reference. Data obtained from different concentration (250, 500, 750, 1000, 1250 and 1500 ppm) of zinc oxide green nanoparticles indicated that the increasing of concentration and exposure period caused increasing in adult mortality (%). Among the different concentration 1500 ppm of zinc oxide nanoparticle proved to be superior by recording highest mortality, lowest number of eggs, least seed damage, and seed weight loss in chick pea seeds up to one month of storage.

Keywords: Callosobruhus analis, Zinc oxide nanoparticles, Chick pea, Mortality

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How to cite this article:

Wazid, Sushila Nadagouda, A. Prabhuraj, R. Harishchanra Naik, N.M. Shakuntala and Sharanagouda, H. 2018. Effect of Biosynthesized Zinc Oxide Green Nanoparticles on Pulse Beetle, Callosobruchus analis (Coleoptera: Chrysomelidae).Int.J.Curr.Microbiol.App.Sci. 7(9): 503-512. doi:
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