Among the stored product insects, Callosobruhus analis (Fabricius) is a cosmopolitan and serious pest of pulses in field as well as in storage condition. The excessive use of pesticides and chemical compounds led to development of resistance, resurgence and has an adverse effect on environment. This experiment was conducted at Centre for Nanotechnology Laboratory, UAS, Raichur. In the recent years, nanotechnology has emerged as a promising tool for pest control. In the present study, we reported the biological synthesis of zinc oxide green nanoparticles from spinach leaves. The green synthesized zinc oxide nanoparticles were characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), Zetasizer and Scanning electron microscope (SEM). The bio-physical characterization revealed that the zinc oxide nanoparticle has a hexagonal structure with a mean particle size of 20.67 nm. The pesticidal effects of Zinc oxide green nanoparticles (ZnO) were used as stored product insect protectants compared to chemical malathion as standard reference. Data obtained from different concentration (250, 500, 750, 1000, 1250 and 1500 ppm) of zinc oxide green nanoparticles indicated that the increasing of concentration and exposure period caused increasing in adult mortality (%). Among the different concentration 1500 ppm of zinc oxide nanoparticle proved to be superior by recording highest mortality, lowest number of eggs, least seed damage, and seed weight loss in chick pea seeds up to one month of storage.

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