National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Aspergillus species have recently caused increasing numbers of acute invasive infections in immunocompromised patients. This prospective and experimental study was carried out at Department of Microbiology and Central Research Laboratory, MGM Medical College and Hospital, Navi Mumbai. Detection of Aspergillus species was done using Polymerase Chain Reaction. This detection level (sensitivity 25%) is higher than Culture (19.5%), Fluorescence (17%) and Microscopy (10%) and difference in sensitivity is statically significant P value (0.001) <0.05, Significant. Species detection in nested PCR was A. niger (50%), A.fumigatus (35%) and A.flavus (15%). This shows more species specificity of PCR. Molecular methods are more sensitive and specific methods. It gives results for detection and speciation in short time which helps early diagnosis and treatment of patient.
Aspergillus, Polymerase Chain Reaction, Sensitivity,Patient
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