National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A biofilm is an assemblage of microbial cells that is irreversibly associated with a surface and enclosed in a matrix of primarily polysaccharide material. A biofilm is an intricate aggregation of microorganisms growing on a solid substrate. It acts as a defensive tool for microorganisms during various stress conditions. The proposed work was carried out to study the biofilm forming ability of microorganisms specifically Pseudomonas aeruginosa five UTI samples different unwell age group persons from Shagun Pathology Lab. The bacteria were isolated from using King’s media which is selective for growth of Pseudomonas Morphological and biochemical characterizations of isolates were carried out. All five isolates were found to be Gram negative chains resembling like Pseudomonas aeruginosa. Biochemical identification was performed. Citrate test was negative for all the isolates. Sugar fermentation using different sugars like Mannitol, lactose, dextrose, maltose and sucrose indicated only acid production, Urease test is positive from all samples. Biofilm forming ability was studied by cover slip essay, dry weight easy, Congo red agar method (CRA), Tube assay (Ring formation in glass surface); Cell surface Hydrophobicity and Effect of carbon source utilization. Different anti-biofilm agents will be further screened for the anti-Biofilm potential.
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