National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The molecular characterization of Clostridium perfringens in sheep for various toxinotypes was investigated in this study. A total of 147 samples collected from healthy, diarrhoeic animals and morbid material of animals suspected to have died of enterotoxaemia were screened for Clostridium perfringens (C. perfringens) toxinotypes. The polymerase chain reaction (PCR) amplification of 16S rRNA gene revealed that out of 147 samples collected, 92 (62.58%) were found positive for C. perfringens. All the 92 isolates were screened for three toxin genes viz., cpa, cpb and etx using a multiplex PCR. Toxinotyping revealed that 65 (72.65%) were positive for Clostridium perfringens Type A and 27 (29.34%) were that of Clostridium perfringens Type D None of the isolates was found to be toxinotype B or C.
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