National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Johne’s disease (JD) is caused by Mycobacterium avium Subsp. Paratuberculosis (MAP) and the disease is characterized by chronic diarrhea, progressive weight loss and emaciation. The present study was carried out to find out the MAP infection status by postmortem and molecular diagnosis in sheep and goats suspected for JD. Postmortem examination of six small ruminants (One sheep and five goats) revealed the pathognomonic necrotic findings such as characterised hypertrophy of mucosal surfaces of small intestine (terminal ileum) and cord like thickening of mesenteric lymphnodes. Acid fast staining of cytological smears prepared from the intestinal scrapings and mesenteric lymphnode of all animals revealed presence of clusters of acid fast bacilli. Mesentric lymphnode and intestine were collected from during postmortem examination and subjected to polymerase chain reaction (PCR) using specific primers targeting IS 900 gene sequence of MAP which revealed positivity to MAP infection. The study concluded that postmortem examination combined with polymerase chain reaction is highly useful in accurate diagnosis of JD in small ruminants.
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