National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pseudomonas aeruginosa is the leading cause of nosocomial infection. The simultaneous detection of MBLs as well as the recent emergence in Pseudomonas aeruginosa of extended-spectrum β-lactamases (ESBLs) with carbapenem-hydrolyzing activity such as the KPC enzymes, add further complexity and concern to the resistance pattern of this organism. So, detection of carbapenemase producers either by phenotypic or molecular tests in the clinical laboratory is of major importance for the determination of appropriate therapeutic schemes and the implementation of infection control measures. The aim of this work is to establish a phenotypic screening test for identification of KPC among Pseudomonas aeruginosa isolates by using carbapenem (Imipenem and Meropenem) disks supplemented with aminophenylboronic acid. Fifty clinical isolates of carbapenem resistant Pseudomonas aeruginosa were collected from different clinical specimens. Phenotypic screening test was made for detection of KPC carbapenamase among Pseudomonas aeruginosa isolates by using of modified hodge test and carbapenem (Imipenem and Meropenem) disks supplemented with aminophenylboronic acid Combined Disk Testing (CDT). Out of 50 Pseudomonas aeruginosa isolates, Bla-KPC gene was detected in 15 (30%) of isolates by real time PCR, 33 (66.0%) were positive by Modified Hodge test with sensitivity 100%. Out of 50 Pseudomonas aeruginosa isolates, CDT using (IPM+APB) and CDT (MEM+APB) positive results were present in 18% and 22% of samples respectively. Based on PCR as a reference test, the sensitivity, specificity, PPV, NPV and accuracy for CDT (IPM+APB) were 53.3%, 97.1%, 88.8%, 82.9%, and 84%. For CDT (MEM+APB) were 66.7%, 97.1%, 90.9%, 87.1% and 88% respectively. This phenotypic screening test is reliable for detecting P. aeruginosa isolates suspected of producing KPC carbapenemases.
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