National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An experiment was conducted for isolation of cellulolytic microorganisms from diverse environments. Samplings were done from 8 locations representing 27 samples viz. forest ecosystem, composting units, incorporation experiments and agricultural fields. A total of 653 microorganisms including bacteria, actinomycetes and fungi were isolated using serial dilution plate assay. All the cultures were initially screened for cellulolytic potential using carboxy methyl cellulose medium on the basis of zone of hydrolysis obtained. Among these cultures, 44 bacteria, 2 actinomycetes and 2 fungi were found to be efficient cellulose degraders. A variation in morphotypes obtained from different samples was found with maximum number of morphotypes were obtained from long term straw incorporation experiments. Among all the 48 cultures highest number of cellulolytic cultures were obtained from forest samples i.e. 35.4% followed by 29.2% from incorporation of crop residues, 25% from compost samples, 6.3% from field soil and 4.2 % from cow dung. Among 48 cultures 21 cultures each produced zones ≤ 10mm and ≤ 20mm, 4 cultures exhibited zones ≤ 30mm and 2 cultures produced highest zones of ≤ 40mm. So overall highest number of efficient cultures was obtained from forest followed by incorporation and compost samples. In nutshell, this study revealed that these systems represent a rich source of cellulolytic microbial biodiversity and needs further research for better understanding synergism between different microorganisms and decomposition process in nature.
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