National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The objective of the present investigation was to isolate and identify the antimicrobial activity showing actinomycetes associated with sponges. Sponges such as Acanthella klethra, Theonella cylendrica, Helicona sp., Auletta sp., and Callyspongia sp. were collected at Sangumal, in Rameswaram island. Ramanathapuram District, Tamil Nadu, India. Isolates of 22 actinomycetes were isolated from sponges. Isolates No. 16 showed high antibacterial and antifungal activity and the isolate was identified as Streptomyces sp. Antibacterial and antifungal activity was determined by agar diffusion method and Minimum Inhibitory Concentration (MIC) of the extract was determined by two fold dilution method using microstate plate. The study showed that fungal pathogens were more susceptible to the extract than bacteria. Among bacterial pathogens, Escherichia coli was more susceptible to the extract, followed by P. aeruginosa, Bacillus subtilis, and Staphylococcus aureus and their growth were inhibited at the concentration of 2 mg/ml. Other bacterial pathogens were resistant to the crude extract derived from culture broth extract of Streptomyces sp. Fungal pathogens such as Candida albicans, Zynomonas sp., Aspergillus niger and A. rutus were susceptible to the extract and the MIC for the fungal pathogens ranged between 0.015 mg/ml and 0.025 mg/ml. A. rutus was susceptible to the extract and it growth was inhibited at the concentration less than 0.1µg/ml. Since the inhibitory concentrations against the fungal pathogens were too less, its purified form may be the potential source for controlling fungal pathogens.
Citations |
 |
 |
 |
 |
