National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The robust technique of polymerase chain reaction (PCR) has revolutionized the field of plant molecular biology and has made the molecular characterization of crop plants easy, rapid and reproducible. Only a minute amount of input genomic DNA is required in PCR. But, the isolated DNA must be free from different contaminants, which can potentially act as PCR inhibitors. The efficiency of PCR might be reduced in case of the medicinal and aromatic plants, due to the presence of biomolecules acting as PCR inhibitors. Hence the present study reports the applicability of a simple and rapid method for isolating genomic DNA from medicinal and aromatic plants, which can be successfully applied for PCR-based genotyping of these plants. Using a modified form of the detergent (SDS)-potassium acetate method, genomic DNA from the mature leaf tissues of 7 different medicinal and aromatic plants, viz., slender dwarf morning-glory (Evolvulus alsinoides L.), horse mint (Mentha longifolia L.), centella (Centella asiatica L.), brahmi (Bacopa monnieri L.), ashwagandha (Withania somnifera L.), vasaka (Adhatoda vasica Nees.) and sarpgandha (Rauwolfia serpentina L.) was isolated and PCR was carried out with different inter short sequence repeat (ISSR) primers. Gel electrophoresis revealed the presence of distinguishable sharp amplicons, prompting us to advocate the utility of this method for genotyping of medicinal and aromatic plants.
Citations |
 |
 |
 |
 |
