National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Present study was conducted on 50 bulls and 40 male calves of Sahiwal, Tharparkar and Karan Fries cattle maintained at Artificial Breeding Research Center and Livestock Research Center, NDRI Karnal (Haryana) to identify genetic polymorphisms in TNP2 gene. A total of 1528 bp of TNP-2 gene were studied in Bos indicus cattle breeds, which are widely distributed in Indian sub-continent. Three sets of primers for TNP2 gene on the basis of Bos Taurus sequence (Acc. No- BK_006514) were designed using Primer3 software and PCR products of 459, 483, 484 base pairs were obtained. Amplicons were custom sequenced and subjected to Clustal W analysis which showed nucleotide changes in non coding region in Indian cattle breeds as compared to Bos taurus. Analysis of SNPs were performed, using restriction fragment length polymorphism (PCR-RFLP), to detect nucleotide changes in the sequence as reported (g. 480 C>T and g. 1536 C>T) in Chinese Holstein breed. Polymorphism at 480 C>T was present while for g. 1536 C>T, there were absence of variability in the sampled population of Sahiwal, Tharparkar and Karan Fries cattle.
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