The prevalence of leaf spots and wilt of transgenic Bt cotton were surveyed in several cotton fields of North Maharashtra region, India. In this study, total four phytopathogenic fungal strains from the affected cotton plants were isolated and characterized. The pathogenicity of fungal strains was confirmed by Koch’s postulates. The pathogenicity tests showed that all the isolated fungal strains are phytopathogen of the Bt cotton plant. Among the four fungal isolates, the prevalence of Fusarium chlamydosporum as potential phytopathogen of cotton is documented for the first time from India.

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