National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was aimed to investigate the modulatory effect of eHSP70 on major heat stressed genes in in-vitro cell culture system. HSP70 (eHSP70) on mRNA expression profile of Heat shock protein 70 (HSP70), Heat shock protein 90 (HSP90), Heat shock protein 110 (HSP110), Interleukin-2 (IL-2), Interleukin-6 (IL-6) in peripheral blood mononuclear cells (PBMC) in cell culture model of black Bengal Goat. The cultured PBMCs were treated with a specific dose of eHSP70 (50 ng/ml) at 37°C for 0, 1, 2, 4, 8, 12, 16, 20, 24 hours. The mRNA expression of the above mentioned genes were determined by quantitative real-time PCR (qRT-PCR). The proteins were immunochemically localized by the cultured PBMCs. The study showed that on administration of eHSP70 to the PBMCs cell culture system, the relative mRNA expression of iHSP70, HSP90 and HSP110 increased significantly (p<0.05) at 4, 20 and 24 h compared with control and Interleukins (IL-2), (IL-6), showed significantly higher expression (p<0.05) at 4 and 24 h compared with control. Immunoreactivity revealed that all genes under study were localized in cytoplasm as well as in nucleus. The differential expression pattern of above mentioned genes at specific dose of eHSP70 might indicate their role in maintaining the cellular homeostasis during heat stress.
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