National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Chlorpyrifos (0, 0 – diethyl 0 - (3, 5, 6 – trichloro – 2 - pyridyl phosphorothioate) is broadspectrum pesticide with trade names Dursban, Lorsban and Spannit, displaying insecticidal activity against Lepidoptera, Diptera, Homoptera, Coleoptera, Orthoptera, Hymenoptera and Hemiptera. However, these organophosphates pesticides have acute neurotoxicity as they suppress acetylcholine esterase (AChE). Hence, present study deals with exploring bacteria for chlorpyrifos degradation ability. Total 10 different samples were collected from soil where chlorpyrifos pesticide was applied. Out of these 10 samples, six samples showed bacteria which are able to grow on Bushnell and Haas agar containing chlorpyrifos as sole carbon source. 15 bacteria which produce zone of clearance near colony were considered efficient chlorpyrifos degrader and purified for further studies. Screening of the isolate for chlorpyrifos revealed that AUC10 showed higher degradation (95 %), followed by AUC12 (93 %) and AUC11 (90 %). Ability of isolates to emulsify hydrophobic molecules revealed that higher emulsification activity was found in groundnut oil followed by coconut oil and cotton seed oil. Morphological characterization revealed that out of 15 isolates 6 bacteria were Gram positive whereas 9 isolates were Gram negative. Thus, isolate AUC10 possessed higher chlorpyrifos degradation rate compared to other isolated bacteria and can be used for bioremediation of chlorpyrifos contaminated sites.
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