The present study aims at exploring the bacteria that contaminate potential antibiotics producing indigenous marine Streptomyces species that stored at 4°C 4. Three organisms isolated from stored Streptomyces strain designated as AIAS-5, AIAS-10 and AIAS-39was subjected for taxonomic characterization and identification as they have moderate to high antibacterial activity. It was found that the strain was closely resembled to Bacillus sp. This identification was further confirmed by the 16S rDNA sequence analysis. The result of BLAST search and phylogenetic analysis showed that the strain AIAS-5 was a type strain of Bacillus pumilus SAFR-032 with 98% similarity as it formed a distinct branch with other strains of this species; AIAS-10 shows 99% similarity with Bacillus amyloliquefaciens, phylogenetic tree build from the forward sequence of this strain from a distinct with separate branch in the tree and Strain AIAS-39 was identified as Bacillus toyonensis with 97% similarity. Strains AIAS-39 may be identified as a new species of Bacillus species as according to 1% difference in sequence similarity is needed to warrant a new species name. To confirm it is a new species further work on its phenotypic characteristics is going on.

Download this article as