Endophytes contribute a major role in biodiversity. They are well known for its uniqueness and antimicrobial properties. In our study endophytic bacteria were isolated from surface sterilized rhizome of Zingiber officinale. Sodium hypochlorite and ethanol were used for surface sterilization. Endophytic bacteria were isolated and purified. DNA of the isolates was amplified by 16 S rDNA and was identified. All the five of them belonged to Bacillus. These isolates were screened for antibacterial activity against a panel of five human pathogens namely Bacillus subtilis, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus and Salmonella typhi by perpendicular streaking. All of the endophytic bacteria except one showed prominent antibacterial activity. Methanolic extracts of these isolates also confirmed potential antibacterial activity.

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