National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the contemporary, application of zinc oxide nanoparticles (ZnO NPs) is growing exponentially in food industry and allied sciences. Hence public concern about their potential adverse effect is increasing. ZnO NPs with their unique physiochemical properties are thought to underlie their exploitable immunological activities. A study was conducted to investigate effects of ZnO NPs with innate immune cells. To achieve this aim, amongst three experimental groups, two group received (5.0 and 50 mg/kg b.w), ZnO NPs was dispensed, subsequent analysis of the body, and different organs weight, cytotoxicity were done using (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazoliumbromide (MTT), Water-soluble tetrazolium salts (WST), Nitric oxide (NO), phagocytosis. The levels of NO yield, phagocytosis, and weight of spleen increased (*P<0.05) due to ZnO NPs at dose of 50 mg/kg b.w., which caused adverse effect on functionality of macrophages. ZnO NPs release of Zn 2+ ions and produced reactive oxygen species and caused dose dependent toxicity. The results demonstrate that the high dose of ZnO NPs caused toxicity which is analysis by body and liver, kidney, and spleen weight, cell viability (MTT and WST), nitric oxide (NO) level, phagocytosis assay. The levels of NO production, phagocytic activity, and spleen weight increased significant (*P< 0.05) while decrease in NO production and phagocytosis was observed. ZnO NPs (5.0 mg/kg b.w) caused significant increased phagocytosis, cell viability, therefore high at dose ZnO NPs caused immunocytotoxicity. Overall, nanotoxicology of ZnO NPs and their effect requires more study to fill up gap of immunotoxicity.
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