The applicability of   repetitive palindromic sequences based differentiation of Pasteurella multocida serogroup B isolates involved in outbreak of haemorrhagic septesemia (HS) in Tarai region of India was evaluated. Seven isolates of Pasteurella multocida B: 2 were isolated from buffaloes and cattle. These were subjected to phenotypic and genotypic characterization. PCR assays were used for the characterization of these isolates. Repetitive extragenic palindromic REP- PCR and Enterobacterial repetitive intergenic consensus ERIC- PCR differentiated all the seven isolates into six profiles. All the isolates were found genetically distinct from standard Pasteurella multocida strain P52 (Vaccine strain of India).Two isolates shared same profile while all other isolates shown different profiles. This study provides a clear evidence of presence of more than one isolate in single outbreak of HS and also provide indication of high  genetic variation among field isolates of Pasteurella and may be the reason of vaccine failure and outbreaks.

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