National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study demonstrated the antibiotic properties of aqueous and methanol extracts of Acalypha ciliata leaves. This was carried out using both gram-negative and gram-positive bacteria which included Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Proteus mirabilis, Klebsiella pneumonia, and Streptococus pyrogenes. The aqueous extract of Acalypha ciliata leaves showed antibacterial activity ranging from 8.00 - 17.00±2.00mm, while that of methanol extract was from 9.00 - 20.00±4.00mm. The duration of antibacterial activity observed in aqueous extract ranged from 5.00 – 11.00± 2.00 days, while that of methanol extract ranged from 5.00 – 12.00±.00 days. The minimum inhibitory concentrations (MIC) of both methanol and aqueous extracts against the test bacteria were between 6.25 – 12.50±0.00mg/ml. However, the minimum bactericidal concentration (MBC) of both aqueous and methanol ranged from 9.38 – 25.00±0.00 mg/ml. The result of colony forming unit (CFU) showed that Klebsiella pnumoniae had the highest CFU value of 4.15 ±0.04 x 108cfu/ml while Proteus mirabilis had the least CFU of 1.27±0.06 x 108cfu/ml. This research indicates that extracts of Acalypha ciliata leaves has broad spectrum antibacterial properties.
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