National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A brief study was carried on fourty Staphylococcus aureus isolates from Bovine milk from Ludhiana, Punjab, India for detecting the antibiotic resistance profile using E-strips and genotypically targeting various antibiotic resistance genes. The antibiotics used for phenotypic resistance detection were Penicillin, Gentamicin, Erythromycin, Clindamycin, Tetracycline, Chloramphenicol, Ciprofloxacin, Trimethoprim-sulphamethoxazole, Oxacillin, Vancomycin andAmoxyclav. The resistance genes targeted were blaZ (Penicillin); ermA, ermB, ermC (Erythromycin); tetK, tetL, tet M, tetO (Tetracycline); aacA-aphD (Gentamycin), mecA (Oxacillin). This study provides a bird view of antimicrobial susceptibility pattern of Staphylococcus aureus in Ludhiana and can help to form scientific basis for field Veterinarians to make therapy decisions empirically thus forming a pillar in combating the antibiotic resistance problem. This study also reiterates the usefulness of PCR based technique for detection of antibiotic resistance accompanying the phenotypic tests.
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