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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:7, July, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(7): 136-142
DOI: https://doi.org/10.20546/ijcmas.2018.707.017


Influence of Different Doses of Benzyl Adenine with Constant Naphthalin Acetic Acid on Callus Induction and Mass Multiplication of Pineapple (Ananas comosus L. Merr) var. Kew
Mayengbam Premi Devi, Kenny Thangjam and R.K. Dilip Singh*
College of Agriculture, Central Agricultural University, Iroisemba-795004, Imphal, Manipur, India
*Corresponding author
Abstract:

A study on influence of different doses of Benzyl Adenine (BA) combined with constant level of Naphthalin acetic acid (NAA) on callus induction, shoot multiplication, and rooting of Kew pineapple was conducted. Early callus response was observed in treatment concentrations of 2mg/l BA with 2mg/l NAA and 3mg/l BA with 2mg/l NAA. Treatment 2mg/l BA with 2mg/l NAA was resulted to replicate the maximum number of shoots (22.71 at 90 days) significantly among the treatments. Shoot length was most enhanced by 0.5 mg/l BA with 2 mg/l NAA (8.09 cm at 90 days) dose. Shoots in control condition developed to be shorter in all the culture periods. On sub culturing in fresh and same media of all the treatments, root initiation was observed 30 days after culture. 0.1 mg/l BA with 2 mg/l NAA was found to be significantly efficient with the number (6.33 at 90 days) and length (2.08 cm at 90 days) of roots developed among the treatments. The plantlets were acclimatised at a satisfactory rate.


Keywords: BA, in vitro, Pineapple, Proliferation, Kew, Manipur

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How to cite this article:

Mayengbam Premi Devi, Kenny Thangjam and Dilip Singh, R.K. 2018. Influence of Different Doses of Benzyl Adenine with Constant Naphthalin Acetic Acid on Callus Induction and Mass Multiplication of Pineapple (Ananas comosus L. Merr) var. Kew.Int.J.Curr.Microbiol.App.Sci. 7(7): 136-142. doi: https://doi.org/10.20546/ijcmas.2018.707.017
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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