Recent advancements in technology has brought to use, plethora of implants or devices made of inert metals, plastics and other synthetic products. Unfortunately, adhesion and attraction of the bacteria to these surfaces made them extremely prone to bacterial biofilm formation. Effects of diabetes, promotes bacterial colonization and allows bacteriuria to easily extend resulting in urosepsis. Present study is designed to provide insight into bacterial colonization and biofilm formation among diabetic patients with indwelling urinary catheters. This prospective study was done on 189 randomly selected urinary catheterized diabetic patients with significant bacteriuria (symptomatic and asymptomatic). Semi-quantitative bacterial culture was performed and isolates were identified by standard biochemical tests. Biofilm formation was Tissue Culture Plate Method. One hundred and two (53.96%) patients had asymptomatic bacteriuria (ASB) and 87/189(46.03%) had urinary tract infection (UTI). Females (57.84%) were frequently seen with ASB than men (42.15%) and ASB was more common in the age group between 46 and 60 years (27.2%).Urinary tract infections on the other hand were more common in males (65.51%) than in females (34.48%).Escherichia coli was most common isolate 59/189(31.12%) followed by Klebsiella spp 50/189(26.45%). Biofilm formation was observed among 140/189 (74.07%) isolates, out of which 55/189(29.10%) were high, 85/189(44.97%) were moderate and 49/189(25.92%) were non biofilm producers. Indwelling bladder catheters are commonly deployed prosthetic medical devices, unfortunately they also provide access for bacteria from contaminated external environment into a vulnerable body cavity. Biofilm-like pods explains how bladder infections can persist in the face of robust host defense. Therefore, very dynamic research activity in the biofilm field is required.

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