National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Medicinal plants have been used as an excellent source for years together as an alternative medicine for treating various disorders including anticancer, cardiovascular disorders and immunological disorders. Pueraria tuberose linn (PT) commonly known as Indian Kudzu is a folklore medicine used in Ayurvedic formulations against various disorders. The present study reports the anticancer potential of the hydroalcoholic tuber extract of PT which is rich in isoflavones. The cytotoxicity of PT tuber extract was evaluated by MTT assay. Further the apoptotic potential of the extract was confirmed by Ethidium bromide/acridine orange (EtBr/AO) staining assay. DNA fragmentation which is considered as the hallmark of apoptosis was also evaluated for the hydroalcoholic tuber extract of PT. The cellular DNA was stained and the distribution of cells in the sub-G1, G0/G1, S and G2/M phases were assessed by flow cytometry. The extract showed a 50% inhibition of cell viability against HT-29 cells at a concentration of 63.91µg/mL. HT-29 cells exhibited apoptotic cell death, increased the expression of caspases, p53 and Bax. Altogether, the results suggest that the hydroalcoholic PT tuber extract has a better anticancer activity against the tested human colon carcinoma cell line.
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