National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
RAPD (random amplified polymorphic DNA) analysis was used to identify molecular markers linked to putative gene conferring resistance to the mustard aphid (Lipaphis erysimi Kalt.). A set of 30 germplasm accessions of Brassica juncea were screened with 284 RAPD primers (87 primers were amplified) for analysis of putative source of aphid resistance. These amplified primers were screened with bulked DNA of tolerant and susceptible accessions and from that four polymorphic primers were obtained. These four primers were screened with individual tolerant and susceptible accessions and only one RAPD primer (oligos-193, Genemed 10-mer) with clear cut polymorphism between tolerant and susceptible accessions was obtained. This polymorphic band of 600 bp found in susceptible was eluted from two accessions i.e. IC-491558 and IC-491564. DNA polymorphisms were converted in to SCAR (Sequence Characterized Amplified Region) primers (BJ 01 and BJ 02). These SCAR primers however could not provide unequivocal result since the association between marker and resistance was not always unidirectional. This indicates the need to further refine the marker identified by primer walking procedure.
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