National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
One of the most important properties of the protease enzyme is to degrade the biofilm because removal of biofilms is very difficult. In industrial settings, both the inactivation and removal of biofilms are of huge concern. If only disinfection without the removal of attached biofilms occurs, the inactivated biofilm cells may provide an ideal environment for further adhesion and growth, resulting in a complex matrix. Microbial resistance to biocides and their negative environmental impact are the main reasons for finding alternative biofilm control strategies. Enzymes are highly selective and capable of distrupting the structural stability of the biofilm EPS (extracellular polymeric substance) matrix. The biofilms acts as a barrier for antibiotics to affect the pathogenic bacteria and this may account for some people developing chronic Borreliosis/ Lyme symptoms. However proteolytic enzymes are very effective at dissolving mucupolysaccharides in biofilm. The 0.2 ml of purified alkaline protease of molecular weight of 29 kDa has been applied for degrading the biofilm of Pseudomonas or E.coli in 10 minutes. When biofilm was treated with the thermophilic protease enzyme the growth of bacteria producing biofilm was minimum (E.coli 0.230 at A 660 nm and Pseudomonas mendocina 0.120 at A 660nm). The combination of protease and polysaccharides was successful in biofilm removal or detachment of the organisms.
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