National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An experiment was carried out at the Agricultural Research Station, Perumallapalle for the regeneration of somaclones. Two sugarcane clones, 2008T42 (susceptible to red rot) and 2009T5 (susceptible to smut) were used as plant material. Calli from the controls and different concentrations of mutagenic chemicals (sodium nitrite and EMS) were transferred on to MS media with 3 mg l-1 BAP, 2 mg l-1 IAA and 2 mg l-1 kinetin for evaluating the effect on shooting. In various treatments, o.6 µM EMS in 2009T5 (T10) exhibited good response for shoot induction as evident by minimum time taken for shoot initiation, more shoot number, frequency and shoot length. Full grown shoots were transferred on to rooting medium with 3 mg l-1 NAA for initiation and elongation of roots to study the effect of mutagenic chemicals on root induction. 0.6 µM EMS in 2009T5 (T10) was found to be better for root induction among all the treatments. It has produced more number of roots with higher length of root in less time.
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