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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
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Original Research Articles                      Volume : 7, Issue:6, June, 2018
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2018.7(6): 1412-1419
DOI: https://doi.org/10.20546/ijcmas.2018.706.168


Assessment of Genetic Polymorphism in Sugarcane Somaclones by Using Random Amplified Polymorphic DNA
S. J. Mallikarjuna*, M. Hemanth Kumar, D. Mohan Reddy, P. Sudhakar and T. Hemalatha
Department of Genetics and Plant Breeding, S.V. Agricultural College, Tirupati, India
*Corresponding author
Abstract:

DNA was extracted from plants and RAPD analysis was performed for fourteen treatments with two random oligonucleotide primers (OPA-01 and OPB-11). Among fourteen treatments, plants derived from the treatments T9, T11, and T12 produced polymorphism T12 was found to exhibit maximum polymorphism at 1.0 µM EMS in 2009T5. The clone, 2009T5 produced more variation than 2008T42. Sodium nitrite did not produce any variation.


Keywords: RAPD, Somaclones, Polymorphism, EMS, Sodium nitrite

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How to cite this article:

Mallikarjuna S. J., M. Hemanth Kumar, D. Mohan Reddy, P. Sudhakar and Hemalatha T. 2018. Assessment of Genetic Polymorphism in Sugarcane Somaclones by Using Random Amplified Polymorphic DNA.Int.J.Curr.Microbiol.App.Sci. 7(6): 1412-1419. doi: https://doi.org/10.20546/ijcmas.2018.706.168
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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