National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Systemic candidiasis is associated with a high crude mortality rate, even with first line antifungal therapy. C. albicans is the predominant cause of invasive fungal diseases which is a serious public health issue. The main objective is to assess the reliability of different media for germ tube production in Candida albicans isolated from various clinical samples. All Candida isolates were identified and speciated by conventional methods such as Gram’s staining, germ tube test, chlamydospore formation on corn meal agar, sugar fermentation test, sugar assimilation test, and growth on Hi-chrome candida agar. Out of 128 clinical isolates of Candida albicans, 5 different methods were used for germ tube production. Pooled human sera showed 121/128 (94.53%) was the most sensitive method wherein YEPD (Yeast Extract Peptone Dextrose) Broth 104/128 (81.25%) was the reliable and easy method for detection of germ tube, followed by Trypticase soy broth 93/128 (72.65%); Peptone water 79/128 (61.71%) and 2% Sucrose 76/128 (59.37%).
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