Molecular Characterization of Trichoderma harzianum and Trichoderma viride Isolated from Rhizosphere Soils by RAPD Markers

S.E. Navyashree; Sumangala Koulagi; S; hyarani Nishani; V. Kantharaju; Chaya P. Patil

doi:https://doi.org/10.20546/ijcmas.2018.706.054

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Original Research Articles Volume : 7, Issue:6, June, 2018

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Int.J.Curr.Microbiol.App.Sci.2018.7(6): 490-497

DOI: https://doi.org/10.20546/ijcmas.2018.706.054

Molecular Characterization of Trichoderma harzianum and Trichoderma viride Isolated from Rhizosphere Soils by RAPD Markers

S.E. Navyashree¹, Sumangala Koulagi¹, Sandhyarani Nishani²,V. Kantharaju³ and Chaya P. Patil⁴

¹Department of Plant Pathology
²Department of Biotechnology and Crop Improvement, K. R. C. College of Horticulture, Arabhavi, Karnataka, India
³Department of Plant Pathology and Head AICRP on Fruits, KRCCH, Arabhavi, Karnataka, India
⁴KRCCH, Arabhavi, University of Horticultural Sciences, Bagalkot-587104, India

^*Corresponding author

Abstract:

Twenty five isolates of Trichoderma harzianum and Trichoderma viride were obtained from rhizosphere soil of turmeric and ginger collected from Belagavi and Bagalkot districts of Karnataka and eight potential isolates based on antagonistic activity were studied using RAPD PCR. The genetic relatedness among two isolates of Trichoderma harzianum and six isolates of Trichoderma viride were analysed with five random primers. RAPD profiles showed genetic diversity among the isolates with the formation of two major clusters. Analysis of dendrogram revealed that similarity coefficient ranged from 0.15 to 0.95. Molecular variability among the potential isolates showed 81 amplified bands out of which 77 were polymorphic and 4 were monomorphic.

Keywords: Trichoderma harzianum, Trichoderma viride, RAPD, Genetic diversity, PCR, Dendrogram

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How to cite this article:

Navyashree, S.E., Sumangala Koulagi, Sandhyarani Nishani, V. Kantharaju and Chaya P. Patil. 2018. Molecular Characterization of Trichoderma harzianum and Trichoderma viride Isolated from Rhizosphere Soils by RAPD Markers.Int.J.Curr.Microbiol.App.Sci. 7(6): 490-497. doi: https://doi.org/10.20546/ijcmas.2018.706.054

Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Original Research Articles Volume : 7, Issue:6, June, 2018

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