National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Spontaneous bacterial peritonitis (SBP) means the development of peritonitis (infection in the abdominal cavity) despite the absence of an obvious source of infection. It occurs almost exclusively in people with portal hypertension, usually as a result of liver cirrhosis, our aim of this study was detection of bacterial infections in SBP by conventional method and molecular biology. (19.5%) ascitic fluid (AF) samples were culture positive. PCR results show that bactDNA was detected in 56 samples (28%), 18 samples were sequenced for molecular identiï¬Âcation of the amplified bactDNA fragments. 10 out of 18 were undetermined, while 6 were E. coli and 2 were Streptococci. Our conclusion is that the detection of bacterial DNA in the ascitic fluid provides an important role in diagnosis of SBP.
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